FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005440641

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005440641
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58469298
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACTTGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA30605155.234396691405462TruSeq Adapter, Index 4 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACTTGGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA6515871.1144087962198554TruSeq Adapter, Index 4 (97% over 37bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGGACTTGGAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA986810.1687740461669302TruSeq Adapter, Index 4 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)