FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005440651

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005440651
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65185563
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGACCTGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA28881194.430611422348227TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACCTGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA10187471.562841453099055TruSeq Adapter, Index 9 (97% over 37bp)
GTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAG1083880.16627608171459685No Hit
GTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT901260.1382606759107074No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGACCTGAAAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA897880.13774215618878677TruSeq Adapter, Index 9 (97% over 36bp)
GGGTGACTTCGCAGGCGTAGACTTTGTGTTTCTCGTAGTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGT696070.10678284699328286No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)