FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005440652

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005440652
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65185563
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCACCAAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAA12283711.8844218619389694Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCACCAAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA7604351.166569658990289Illumina Single End PCR Primer 1 (96% over 32bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTCACCAAGTGTAGATCTCGGTGGTCGCCGGATCATTAAAAAAA1262920.1937422861562153Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)