FastQCFastQC Report
Thu 16 Jun 2022
EGAF00005440656

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005440656
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47171766
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACGTCTTGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAA10599402.246979686959356Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACGTCTTGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA6440141.3652531050035313Illumina Single End PCR Primer 1 (96% over 32bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGACGTCTTGTGTAGATCTCGGTGGTCGCCGGATCATTAAAAAAA914550.19387656591020994Illumina Single End PCR Primer 1 (96% over 31bp)
CCTAGACCAAACCTACGCCAAAATCCATTTCACTATCATATTCATCGGCGTAAATCTAACTTTCTTCCCACAACA575060.12190766824375411No Hit
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGAGCTGGAGTCCTAG493520.1046219045519729No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)