FastQCFastQC Report
Thu 12 May 2022
EGAF00005440658

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005440658
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57222088
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGCGAGACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAA3907720.6829041261129793Illumina Single End PCR Primer 1 (96% over 33bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGCGAGACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA3878500.6777977063682122Illumina Single End PCR Primer 1 (97% over 34bp)
GTTGACTATTCTCTACAAACCACAAAGACATTGGAACACTATACCTATTATTCGGCGCATGAGCTGGAGTCCTAG824430.14407548357899838No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAATGACCTCCG765440.13376652735915545No Hit
CCTAGACCAAACCTACGCCAAAATCCATTTCACTATCATATTCATCGGCGTAAATCTAACTTTCTTCCCACAACA744100.13003719822317564No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGCGAGACGTGTAGATCTCGGTGGTCGCCGGATCATTAAAAAAA582070.10172120947421562Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)