FastQCFastQC Report
Thu 12 May 2022
EGAF00005440667

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005440667
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50000450
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGGAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA2713040.5426031165719509TruSeq Adapter, Index 18 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGGAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1540990.3081952262429638TruSeq Adapter, Index 18 (97% over 38bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)