FastQCFastQC Report
Thu 12 May 2022
EGAF00005440669

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005440669
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57155779
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA23759574.156984720652657TruSeq Adapter, Index 16 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTGAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA9572161.6747492847573646TruSeq Adapter, Index 16 (97% over 37bp)
GGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGTCCGTGGAAGCCTGTGGCTACAAAAAATG1246230.2180409438562634No Hit
GGAAAGTTGAGCCAATAATGACGTGAAGTCCGTGGAAGCCTGTGGCTACAAAAAATGTTGAGCCGTAGATGCCGT992330.17361848921698714No Hit
GCCGTAGATGCCGTCGGAAATGGTGAAGGGAGACTCGAAGTACTCTGAGGCTTGTAGGAGGGTAAAATAGAGACC902750.15794553338167258No Hit
GCCAGTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAAATATTAGTTGACGGATGAAGC867550.1517869260429466No Hit
GTCGGAAATGGTGAAGGGAGACTCGAAGTACTCTGAGGCTTGTAGGAGGGTAAAATAGAGACCCAGTAAAATTGT755370.13215986435947272No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTGAAGAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA752920.13173121129186255TruSeq Adapter, Index 16 (97% over 36bp)
GCGGCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAAATATTAGTTGACGGATGAAGCAGATAGTGAGG746900.1306779494685918No Hit
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATGTGCTTTCTCGTGTTACATCGCGCCA722370.12638616997941712No Hit
GTAAAGTGAAATATTAGTTGACGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGTCCGTGG676580.11837473162600058No Hit
CCAGTATCAGGCGGCGGCTTCGAAGCCAAAGTGATGTTTGGATGTAAAGTGAAATATTAGTTGACGGATGAAGCA629240.11009210459715718No Hit
GTGAAATATTAGTTGACGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGTCCGTGGAAGCC612150.10710203075003143No Hit
GGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGCCGAGAAAGTGTTGTGGGAAGAAAGT582590.10193020026898766No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)