FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005440670

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005440670
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57155779
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATCCACTGGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAA16278752.848137193616065Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATCCACTGGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA8760511.5327426470733607Illumina Single End PCR Primer 1 (96% over 33bp)
CCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTTTTGTAGCCACAGGCT1263290.22102576889031644No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAATGACCTCCG635330.11115761365093108No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)