FastQCFastQC Report
Thu 12 May 2022
EGAF00005440687

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005440687
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61527290
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGCAAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA46499587.557553729410153TruSeq Adapter, Index 16 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGCAAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA22952953.730531606381494TruSeq Adapter, Index 16 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGCAAGCATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAG2310120.3754626605527401TruSeq Adapter, Index 16 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGCAAGCATCTCGTATGCCGTCTTCTGCTTGAGATCGGAAGA2059870.3347896518764275TruSeq Adapter, Index 16 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGCAAGCAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA1253220.20368522650680698TruSeq Adapter, Index 16 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)