FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005440688

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005440688
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61527290
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGGCGTGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA31413285.105584855110635Illumina Single End PCR Primer 1 (97% over 34bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGGCGTGAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAA17467902.839049143883958Illumina Single End PCR Primer 1 (96% over 33bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGGCGTGAGTGTAGATCTCGGTGGTTGCCGTATCATTAAAAAA796880.12951651210381604Illumina Single End PCR Primer 1 (97% over 34bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGGCGTGAGTGTAGATCTCGGTGGTCGCCGGATCATTAAAAAAA659500.10718820867943314Illumina Single End PCR Primer 1 (96% over 33bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)