FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005440695

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005440695
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64890758
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA10379051.5994650578746514TruSeq Adapter, Index 21 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTCCAATATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA6850371.0556772969118344TruSeq Adapter, Index 21 (97% over 38bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)