FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005440697

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005440697
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66341208
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAACAGGTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA23504263.542935184418107TruSeq Adapter, Index 1 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAACAGGTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA5928500.8936376316813526TruSeq Adapter, Index 1 (97% over 37bp)
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGC1092250.16464125886884665No Hit
GTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGCT914290.13781630265158873No Hit
GTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAG877600.13228580341799023No Hit
GTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT675780.10186428923633709No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)