FastQCFastQC Report
Thu 12 May 2022
EGAF00005440699

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005440699
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51770303
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGCTCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA6727261.2994438143427516TruSeq Adapter, Index 16 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGCTCTAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA6724321.2988759212013883TruSeq Adapter, Index 16 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)