FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005440705

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005440705
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64845193
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATGAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA27922984.306098680283055TruSeq Adapter, Index 7 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATGAGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA7789211.201200835349507TruSeq Adapter, Index 7 (97% over 35bp)
GGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGTCCGTGGAAGCCTGTGGCTACAAAAA1086960.1676238360490345No Hit
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATGTGCTTTCTCGTGTTACATCGCGCCA1038410.16013677374666768No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATGAGAAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA858370.13237218678645926TruSeq Adapter, Index 7 (97% over 34bp)
GGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGCCGAGAAAGTGTTGTGGGAAGAAAGT845500.1303874598692304No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)