FastQCFastQC Report
Thu 12 May 2022
EGAF00005440706

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005440706
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64845193
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAATGCCTCGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAA9488161.4632017519016405Illumina Single End PCR Primer 1 (96% over 32bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAATGCCTCGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA4955940.7642725344344338Illumina Single End PCR Primer 1 (96% over 33bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAATGCCTCGTGTAGATCTCGGTGGTCGCCGGATCATTAAAAAAA1171030.18058855958683012Illumina Single End PCR Primer 1 (96% over 32bp)
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAATGACCTCCG962330.14840421555997219No Hit
CCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTTTTGTAGCCACAGGCT786380.1212703615517036No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)