FastQCFastQC Report
Thu 12 May 2022
EGAF00005440707

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005440707
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64350901
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCATAATACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA20112173.125390583109318TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATAATACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA9427131.4649569553035473TruSeq Adapter, Index 7 (97% over 37bp)
GGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGCCGAGAAAGTGTTGTGGGAAGAAAGT946590.14709817349721335No Hit
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATGTGCTTTCTCGTGTTACATCGCGCCA910170.14143857908065655No Hit
GGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGTCCGTGGAAGCCTGTGGCTACAAAAA819030.1272756072211017No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)