FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005440709

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005440709
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63173672
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCGGCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA13475502.1330879737369073TruSeq Adapter, Index 11 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCGGCGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA9939001.573281983671932TruSeq Adapter, Index 11 (97% over 36bp)
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATGTGCTTTCTCGTGTTACATCGCGCCA1263390.1999867919661216No Hit
GGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGTCCGTGGAAGCCTGTGGCTACAAAAA951940.15068619091826732No Hit
GCCGTAGATGCCGTCGGAAATGGTGAAGGGAGACTCGAAGTACTCTGAGGCTTGTAGGAGGGTAAAATAGAGACC664670.1052131337244414No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)