FastQCFastQC Report
Thu 16 Jun 2022
EGAF00005440718

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005440718
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67072009
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGGACAACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAA8386161.250321874211342Illumina Single End PCR Primer 1 (96% over 33bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCGGACAACGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA4564400.6805223323488043Illumina Single End PCR Primer 1 (97% over 34bp)
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAATGACCTCCG1432000.21350187974837612No Hit
CCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTTTTGTAGCCACAGGCT1193750.1779803554117486No Hit
GTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAATGACCTCCGGCCTAGCCATGTGATTT928680.13846014363458234No Hit
ATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAATGACCTC801950.11956552546383396No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)