FastQCFastQC Report
Thu 12 May 2022
EGAF00005440719

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005440719
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59679801
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGTCGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA10811331.811555973519416TruSeq Adapter, Index 21 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGTCGGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA4982820.8349257062703678TruSeq Adapter, Index 21 (97% over 40bp)
GGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGTCCGTGGAAGCCTGTGGCTACAAAAA660180.11062034204839255No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)