FastQCFastQC Report
Sun 5 Jun 2022
EGAF00005440722

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005440722
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47181818
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATATGGATGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAA16088853.4099682212330182Illumina Single End PCR Primer 1 (97% over 35bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATATGGATGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA4122110.8736649359293447Illumina Single End PCR Primer 1 (97% over 36bp)
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAATGACCTCCG708220.15010443217766642No Hit
CCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTTTTGTAGCCACAGGCT506440.10733795802442374No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTATATGGATGTGTAGATCTCGGTGGTCGCCGGATCATTAAAAAAA488200.10347206205576903Illumina Single End PCR Primer 1 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)