FastQCFastQC Report
Sun 5 Jun 2022
EGAF00005440725

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005440725
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44949102
Sequences flagged as poor quality0
Sequence length75
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTCACCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA22399264.983249720984414TruSeq Adapter, Index 16 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTCACCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA7931881.7646359208688975TruSeq Adapter, Index 16 (97% over 38bp)
GGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGTCCGTGGAAGCCTGTGGCTACAAAAA963730.21440472826353682No Hit
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATGTGCTTTCTCGTGTTACATCGCGCCA620940.13814291551364027No Hit
GGAAAGTTGAGCCAATAATGACGTGAAGTCCGTGGAAGCCTGTGGCTACAAAAAATGTTGAGCCGTAGATGCCGT528780.11763972503833336No Hit
GCCGTAGATGCCGTCGGAAATGGTGAAGGGAGACTCGAAGTACTCTGAGGCTTGTAGGAGGGTAAAATAGAGACC498210.11083869929147863No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTTCACCAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA488430.10866290498973705TruSeq Adapter, Index 16 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)