FastQCFastQC Report
Thu 16 Jun 2022
EGAF00005440726

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005440726
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44949102
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGGAGCGTCGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAA6798101.5123995135653656Illumina Single End PCR Primer 1 (97% over 34bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGGAGCGTCGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA4782051.063881098225277Illumina Single End PCR Primer 1 (97% over 35bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGGAGCGTCGTGTAGATCTCGGTGGTCGCCGGATCATTAAAAAAA996300.22165070172035917Illumina Single End PCR Primer 1 (97% over 34bp)
CCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTTTTGTAGCCACAGGCT589380.1311216406503516No Hit
CACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAATGACCTCCG464840.10341474675066925No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)