FastQCFastQC Report
Wed 11 May 2022
EGAF00005440728

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005440728
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46610684
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTTCCAATGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAA11734742.517607336549706Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGTTCCAATGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA5968731.280549755502408Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)