FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005440729

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005440729
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55472685
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCGAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA8229601.4835409535341582TruSeq Adapter, Index 11 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCGAGACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA4028540.7262204813053488TruSeq Adapter, Index 11 (97% over 36bp)
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATGTGCTTTCTCGTGTTACATCGCGCCA767080.13828066912571477No Hit
GGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGTCCGTGGAAGCCTGTGGCTACAAAAA696190.1255014066833073No Hit
GGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGCCGAGAAAGTGTTGTGGGAAGAAAGT602520.10861561865988639No Hit
GTTGGTTAGTAGGCCTAGTATGAGGAGCGTTATGGAGTGGAAGTGAAATCACATGGCTAGGCCGGAGGTCATTAG555360.10011413725511933No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)