FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005440733

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005440733
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62896906
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCATAGAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA9835931.5638177814342729TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATAGAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA4526810.7197190271966637TruSeq Adapter, Index 7 (97% over 37bp)
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATGTGCTTTCTCGTGTTACATCGCGCCA1029600.16369644637209976No Hit
GGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGTCCGTGGAAGCCTGTGGCTACAAAAA974030.1548613535934502No Hit
GGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGCCGAGAAAGTGTTGTGGGAAGAAAGT819810.13034186451079166No Hit
GCCGTAGATGCCGTCGGAAATGGTGAAGGGAGACTCGAAGTACTCTGAGGCTTGTAGGAGGGTAAAATAGAGACC651870.10364102806583206No Hit
GTTGGTTAGTAGGCCTAGTATGAGGAGCGTTATGGAGTGGAAGTGAAATCACATGGCTAGGCCGGAGGTCATTAG643390.10229279004598413No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)