FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005440734

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005440734
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62896906
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGTGGCAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAA7399641.1764712241966242Illumina Single End PCR Primer 1 (96% over 33bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGTGGCAGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA4406310.700560692126891Illumina Single End PCR Primer 1 (97% over 34bp)
CCTCAGAGTACTTCGAGTCTCCCTTCACCATTTCCGACGGCATCTACGGCTCAACATTTTTTGTAGCCACAGGCT833910.13258362819945388No Hit
GTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAATGACCTCCGGCCTAGCCATGTGATTT736780.11714089720088934No Hit
CGCATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAACAGGGGCCCTCTCAGCCCTCCTAATGACCTCCG723020.11495319022528708No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)