FastQCFastQC Report
Tue 28 Jun 2022
EGAF00005440923

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005440923
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences74883262
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA2305000.3078124454567698TruSeq Adapter, Index 3 (100% over 63bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA918640.12267627978065378TruSeq Adapter, Index 3 (100% over 63bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)