FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005440941

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005440941
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28391866
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG84600.027.3975871
TCGTATG92100.025.09043944
CTCGTAT90300.024.33027843
CGTATGC95350.024.23160445
TATGCCG94700.024.18037447
GATCGGA104050.022.331352
GTCACGA107300.021.6023830
TCTCGTA104450.021.26682342
ACGATCA112250.020.55815533
ATCGGAA113400.020.4178663
ACACGTC114400.020.3768614
AGTCACG114850.020.11966929
ACGTCTG120500.019.40270816
TCACGAT123450.019.05572131
CACGATC125550.018.65456632
ATCTCGT119550.018.35124841
ATGCCGT131300.017.80778348
CGATCAG128650.017.80338934
CACGTCT131600.017.60890215
GTATGCC136500.017.15465546
Produced by FastQC (version 0.11.5)