FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005440945

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005440945
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26567537
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG40600.023.7012443
CGTATGC42600.022.34549344
CTCGTAT42350.022.23500842
TATGCCG46900.020.22116546
GATCGGA53450.019.3831271
ACGGCTA54350.017.70676632
ACACGTC60750.016.6315413
TCTCGTA58500.016.33373541
AGTCACG58750.016.14269628
ATCGGAA65200.016.0393942
ACGTCTG65300.015.26159515
ATCTCGT66900.014.17981740
CGGCTAC70600.013.87582633
CGACCGT17100.013.5762271
CACGTCT76600.013.550314
GTATCGA25500.013.2980764
CGTATCG23700.013.2893143
GTCACGG74000.013.23597129
ATGCCGT77200.013.22271647
CCCGTAT29450.012.8245331
Produced by FastQC (version 0.11.5)