FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005440946

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005440946
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26567537
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCA26500.018.8759350
GCCGTAT26600.018.41600448
GTATCGA27000.016.120854
CGACCGT16350.016.0652831
CCCGTAT30800.015.8219831
CGTATCG25950.015.7143663
GCGTCGT36300.013.98307812
GATCGGA41300.012.1331232
TGGTCGC41900.011.60737143
CGCCGTA44700.011.49919547
CGCGTAT3702.3682242E-611.2091331
TATCGAA41350.011.1915755
CCGTATC48350.010.91644549
ATCGCGC33450.010.72836467
GTCGCCG48800.010.60292445
ATCGAAG54200.010.5134826
AGATCGG48950.010.2378281
AGCGTCG49650.010.15396211
TATATCG18800.010.08416235
GTAGATC55500.09.56244932
Produced by FastQC (version 0.11.5)