FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005440981

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005440981
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69037298
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGCAAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA39436205.71230351454369TruSeq Adapter, Index 16 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGCAAGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA27006233.911831833279454TruSeq Adapter, Index 16 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGCAAGCATCTCGTATGCCGTCTTCTGCTTGGATCGGAAGAG11637781.6857235635148986TruSeq Adapter, Index 16 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGCAAGCATCTCGTATGCCGTCTTCTGCTTGGATCGGAAGA7337891.06288777408409TruSeq Adapter, Index 16 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCGCAAGCATCTCGTATGCCGTCTTCTGCTTGGAACGGAAGAG896240.12981968094985408TruSeq Adapter, Index 16 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)