FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005440985

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005440985
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66783617
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACACCTTGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA11004801.647829287233724TruSeq Adapter, Index 8 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACCTTGGCATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA4086110.6118431710579557TruSeq Adapter, Index 8 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)