FastQCFastQC Report
Thu 12 May 2022
EGAF00005441002

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005441002
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69897193
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGATTCTGCGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAA35494705.078129532326141Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGATTCTGCGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA21396473.061134372019775Illumina Single End PCR Primer 1 (96% over 32bp)
ATCGGAAGAGCGGCGTGTAGGGAAAGAGTGTGATTCTGCGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAA819660.11726651168953238Illumina Single End PCR Primer 1 (96% over 31bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)