FastQCFastQC Report
Tue 28 Jun 2022
EGAF00005441028

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005441028
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81005479
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAACAATGGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAA7786930.9612843595431365Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAACAATGGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA2571310.3174242078119185Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)