FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005441042

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005441042
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75506348
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTTGCCTAGGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAA6764980.8959485101835412Illumina Single End PCR Primer 1 (96% over 31bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTTGCCTAGGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA2000760.2649790452055766Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)