FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005441061

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005441061
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40480100
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA9717692.4006091882184086TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGGCGTGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA5050821.2477291311039251TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA834600.044.8873561
TCGTATG1599900.044.40714644
TATGCCG1603800.044.2928947
CGTATGC1609750.044.13542645
ACCGGCG1614100.044.1347631
CGTGAAT1581500.044.0737836
CTCGTAT1602400.043.991543
GTATGCC1626150.043.6606146
CCGGCGT1634950.043.5824732
CGGCGTG1636800.043.536333
GCGTGAA1644250.043.28980635
AATCTCG1598150.043.2759440
TCACCGG1653400.043.0668829
ATGCCGT1658550.042.81003648
GCCGTCT1653550.042.80824750
GTCACCG1666450.042.7027128
GGCGTGA1672400.042.63224434
TCTCGTA1624850.042.62353542
CACCGGC1674650.042.52245330
CGTCTGA1680800.042.34313615
Produced by FastQC (version 0.11.5)