FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005441062

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005441062
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40480100
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACAGGCGCGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAA9820592.426029085896527Illumina Single End PCR Primer 1 (97% over 34bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACAGGCGCGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA5070391.252563605327062Illumina Single End PCR Primer 1 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA829600.044.97581
CGCCGTA1588700.044.41854557
GCGTGTA1603050.044.32048838
CGTATCA1596600.044.28806360
GCCGTAT1595350.044.2772458
CGCGTGT1610700.044.1080137
GGCGCGT1611450.044.0903935
CCGTATC1608350.043.92590359
TCTCGGT1620250.043.7526547
GTAGATC1626150.043.60778842
AGGCGCG1631200.043.60096734
GGTCGCC1623300.043.5771554
TAGATCT1630250.043.4766943
GTATCAT1629150.043.40737561
GCGCGTG1642200.043.26867736
GAGTGTA1648950.043.06441526
TGGTCGC1642950.043.0475653
TCGGTGG1652450.043.00221349
GTCGCCG1646050.042.96638555
AGTGTAC1656950.042.8835627