FastQCFastQC Report
Thu 12 May 2022
EGAF00005441065

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005441065
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59740822
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTAAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA26275384.398228735453288TruSeq Adapter, Index 15 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTAAGTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA8175091.3684260989914065TruSeq Adapter, Index 15 (97% over 41bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)