FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005441071

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005441071
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63214278
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATCCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA21678813.4294166896915277TruSeq Adapter, Index 3 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCATCCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA8405021.3296078458730478TruSeq Adapter, Index 3 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)