FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005441073

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005441073
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57299474
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCACCAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA18790653.2793756536054763TruSeq Adapter, Index 1 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCACCAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA7755161.3534434888529692TruSeq Adapter, Index 1 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTCACCAAAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA1625090.28361342374626336TruSeq Adapter, Index 1 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)