FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005441115

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005441115
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59616254
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAGAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA16728482.8060266919823573TruSeq Adapter, Index 13 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAGAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA14402642.4158914781864693TruSeq Adapter, Index 13 (97% over 38bp)
GTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGATGGGAG1588600.26647095270360327No Hit
GTGAGGTGAAAGATGAGCTGGAGGACCGCAATAGGGGTAGGTCCCCTGTGGAAAAAGGGTCAGAGGCCAAAGGAT1284540.21546808358673458No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGCAGAGAACTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA894720.15007987586741026TruSeq Adapter, Index 13 (97% over 37bp)
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGC722450.12118339404552322No Hit
GGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTTCCAGATTTCAACTGCTCATCAGATGG706290.1184727238984187No Hit
GTCTGCTTTGCTCAGCGTCAGGGTGCTGCTGAGGCTGTAGGTGCTGTCCTTGCTGTCCTGCTCTGTGACACTCTC688420.11547521922460945No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)