FastQCFastQC Report
Sat 4 Jun 2022
EGAF00005441116

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005441116
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59616254
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGGCATTCTGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAA11863281.9899405286350262Illumina Single End PCR Primer 1 (96% over 33bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGGCATTCTGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA10354411.7368434454133934Illumina Single End PCR Primer 1 (97% over 34bp)
GGGAGAAGTGCCCCCACCTGCTCCTCAGTTCCAGCCTGACCCCCTCCCATCCTTTGGCCTCTGACCCTTTTTCCA774700.12994778236150162No Hit
AGTGCCCCCACCTGCTCCTCAGTTCCAGCCTGACCCCCTCCCATCCTTTGGCCTCTGACCCTTTTTCCACAGGGG671710.11267229235838938No Hit
GTTAGAGGGAGAAGTGCCCCCACCTGCTCCTCAGTTCCAGCCTGACCCCCTCCCATCCTTTGGCCTCTGACCCTT636180.10671250830352408No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)