FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005441125

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005441125
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57280523
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCATTCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA9562901.6694854549425115TruSeq Adapter, Index 23 (97% over 39bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCCATTCGAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA8735481.5250349582178222TruSeq Adapter, Index 23 (97% over 38bp)
GGTTTATTGAGTGCAGGGAGAAGGGCTTGATGCCTTGGGGTGGGAGGAGAGACCCCTCCCCTGGGATCCTGCAGC760600.13278510044330427No Hit
GGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGCCGAGAAAGTGTTGTGGGAAGAAAGT677370.11825485601798713No Hit
GTCGGAGTCCAGCACGGGAGGCGTGGTCTTGTAGTTGTTCTCCGGCTGCCCATTGCTCTCCCACTCCACGGCGAT586270.10235067162358137No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)