FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005441131

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005441131
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49327853
Sequences flagged as poor quality0
Sequence length75
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTGTGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA23921354.849461013435959TruSeq Adapter, Index 12 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTGTGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA15760683.1950873677798217TruSeq Adapter, Index 12 (97% over 37bp)
TATCGGAAGAGCACACGTCTGAACTCCAGTCACATTGTGAAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA1160990.23536195666168563TruSeq Adapter, Index 27 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)