FastQCFastQC Report
Thu 12 May 2022
EGAF00005441150

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005441150
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56692119
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGCTCGCTGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAA12701062.240357253183639Illumina Single End PCR Primer 1 (97% over 37bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGCTCGCTGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAA8491211.4977760841855285Illumina Single End PCR Primer 1 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)