FastQCFastQC Report
Wed 11 May 2022
EGAF00005441165

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005441165
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48363098
Sequences flagged as poor quality0
Sequence length75
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTACTCATAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAA12848922.6567611528938864TruSeq Adapter, Index 7 (97% over 35bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTACTCATAATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAA5290191.093848454455916TruSeq Adapter, Index 7 (97% over 34bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)