FastQCFastQC Report
Fri 27 May 2022
EGAF00005441173

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005441173
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50593358
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTTAGAAATCTCGTATG591500.116912579710562TruSeq Adapter, Index 2 (97% over 36bp)
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA577840.11421262055782105No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)