FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005441197

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005441197
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21479470
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA388840.18102867528854297No Hit
CCCGTCCCGGGGGTTGGCCGCGCGGGCCCCGGTGGGGCGGCCACCCGGGG304730.14187035341188586No Hit
GGCGTACGGAAGACCCGCTCCCCGGCGCCGCTCGTGGGGGGCCCAAGTCC255580.11898803834545266No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCAGAATTATCTCGTATG230220.10718141555634286TruSeq Adapter, Index 5 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTACG51900.070.131972
CGTACGG55700.065.33753
GGCGTAC57650.063.4488451
ACCGCGT29900.052.1278886
CGCGTTC33450.047.029088
CCGCGTT33550.046.6728257
GTACGGA85050.042.7889144
TACGGAA93650.038.9370545
AGACCGC42150.037.1499334
GACCGCG43500.036.3303955
CAGACCG49850.033.1576463
ACGGAAG118300.031.0689146
TCGACCC79800.030.7109682
GACCCGT82700.029.4534154
GCGTTCT54200.029.0244059
CGTGCGG92400.026.7537868
ACCCGTG95000.026.0978895
GTCGCGC46750.025.871691
ATATCGC31150.024.6689518
GCGCCGT48650.024.4378434
Produced by FastQC (version 0.11.5)