FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005441198

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005441198
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21479470
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC543230.25290661268643966No Hit
GTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGACC295460.13755460446649753No Hit
GCTGCAGGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGC287370.13378821730703785No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCC217050.038.4544643
GGTCGAC223800.036.7696881
CCGTGCG226950.036.617818
CGTGCGG228250.036.4409949
CGACCCG229300.036.3997544
ACCCGTG231700.036.0860946
AGGTCGA126150.035.6093946
GTCGACC243000.034.2103772
ACGTGCG59500.028.8596128
GACCCGT301850.028.5871355
TATCGCG24200.027.5442899
CGTGCGC71650.024.8758569
GGCGCGT37600.024.0864164
CCCGTGC392050.021.4375747
GTCTAAC94450.020.7666031
AACACGT87650.020.2518275
TAACACG88350.019.9273594
CGCGTGC50400.018.400926
TGCAGGT258400.018.3374633
GTGCGGA253700.017.8206399
Produced by FastQC (version 0.11.5)