FastQCFastQC Report
Fri 1 Jul 2022
EGAF00005441200

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005441200
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32462076
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCA500960.1543216151671877No Hit
GGTCGACCCGTGCGGAGGAGCGAGGAGGAAGGACGCGCGAGGGCCGGGAC407160.12542635905356145No Hit
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACCTTGGCGTGTAGATCTC350160.10786740811031309Illumina Single End PCR Primer 1 (96% over 32bp)
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG341590.10522740443340715No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)